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Bsu DNA Polymerase (Large Fragment)

Bsu DNA Polymerase (Large Fragment)

$320.00 - $1,280.00
$1,600.00
Bsu DNA Polymerase, derived from Bacillus subtilis, was obtained by truncating the first 296 AAs of the Bacillus subtilis DNA polymerase (Bsu) I gene. This polymerase retains its 5’→ 3’ DNA polymerase activity with its 5’→ 3’ exonuclease domain removed. Bsu DNA Polymerase naturally lacks the 3’→ 5’ exonuclease activity, which can be used for recombinase amplification.
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All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: BSU-500 (for 500U)

Cat. No.: BSU-2500 (for 2500U)

 

The lyophilized version is also available by inquiry, which can be transported at room temperature.

 

Applications

  • Primer labeling
  • Second strand cDNA synthesis
  • DNA strand displacement synthesis
  • Single dA tailing

 

Heat Inactivation

75°C, 20 min.

 

Unit Definition

One unit is defined as adding 10 nmol acidic refractory substance in 30 min at 37°C.

 

Storage

The minimum shelf life is 2 years at -20°C. Avoid repeated freezing and thawing.

 

Notes

  • Due to the lack of the 3’→ 5’ exonuclease activity, Bsu DNA Polymerase (Large Fragment) cannot excise 3' protruding ends and is therefore not suitable for generating blunt ends.
  • At 25°C, Bsu DNA Polymerase (Large Fragment) retains 50% activity, which is twice as high as the Klenow fragment (3´→5´ exo-) at the same temperature.

 

Reagents for Recombinase Polymerase Amplification (RPA)

  • T4 UvsX Recombinase
  • T4 UvsY Protein
  • T4 Gene 32 Protein
  • Bsu DNA Polymerase
  • Sau DNA Polymerase
  • Exonuclease III
  • Bst DNA/RNA Polymerase
  • Endonuclease IV

 

Instruction: RPA Reaction System (for reference only)

 

RPA Lyophilized Kits

In addition to the previously mentioned RPA raw material enzymes, we present a varied range of kits meticulously crafted to cater to your specific requirements. All these kits are lyophilized, ensuring stability during transportation at room temperature. Delve into our exceptional selection:

Gen-1

  • RPA Basic Kit (Gen-1), 
  • RPA RT Basic Kit (Gen-1), 
  • RPA Fluorescent Kit (Gen-1), 
  • RPA RT Fluorescent Kit (Gen-1)

Gen-2 (featuring the additional introduction of a sucrose-sucrose phosphorylase energy system for enhanced stability and reliability in the amplification process)

  • RPA Basic Kit (Gen-2), 
  • RPA RT Basic Kit (Gen-2), 
  • RPA Fluorescent Kit (Gen-2), 
  • RPA RT Fluorescent Kit (Gen-2)

Gen-3 (featuring the additional introduction of the dUTP-UDG-UGI system to effectively prevent aerosol contamination)

  • RPA Basic Kit (Gen-3), 
  • RPA RT Basic Kit (Gen-3), 
  • RPA Fluorescent Kit (Gen-3), 
  • RPA RT Fluorescent Kit (Gen-3)

Our kits are meticulously crafted to deliver accurate and efficient results for your research endeavors. For further information or if you're interested in any of our products, please don't hesitate to contact us. Elevate your work with our cutting-edge solutions today!

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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    Recombinase Polymerase Amplification

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