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We are proud to introduce our groundbreaking RPA Multiplex Fluorescent Lyophilized Kit (Gen-4). This revolutionary exclusive multiplex Recombinase Polymerase Amplification (RPA) kit is capable of completing direct amplification of DNA/RNA within just 10 to 20 minutes.

Cat. No.: RIRB2-48 (for 48T)

Cat. No.: RIRB2-480 (for 480T)

Description

This product represents an upgraded version of RPA (Recombinase Polymerase Amplification) nucleic acid amplification reagents, featuring the additional introduction of a sucrose-sucrose phosphorylase energy system for enhanced stability and reliability in the amplification process. Supplied as a ready-to-use lyophilized reagent, it includes all components except primers, probes, Mg²⁺, and the template.

To use, simply dissolve the RPA lyophilized particles in RNase-free ddH₂O, add the template, primers, and Mg²⁺. The final reaction volume is 25 µL. Under constant temperature conditions of 39 to 42°C, the system specifically recognizes and amplifies nucleic acid fragments of the target sample. Detection and assessment can be performed in conjunction with gel electrophoresis.

Ideal for rapid RNA and DNA amplification and detection.

Composition

Reverse transcriptase, Recombinase, Polymerase, NFO, dNTP, Buffer, DTT, as well as additives such as trehalose, PEG, sucrose, sucrose phosphorylase, and protective agents.

Features

• Enhanced stability and reliability with sucrose-sucrose phosphorylase energy system
• Detection limits closely approaching PCR

Storage

This product is transported and stored at 2~28°C, in a dry, and light-protected environment, with a shelf life of 1 year.

Related:

• Gen-1: RPA Basic Kit (Gen-1), RPA RT Basic Kit (Gen-1), RPA Fluorescent Kit (Gen-1), RPA RT Fluorescent Kit (Gen-1)
• Gen-2: RPA Basic Kit (Gen-2), RPA RT Basic Kit (Gen-2), RPA Fluorescent Kit (Gen-2), RPA RT Fluorescent Kit (Gen-2)
• Gen-3: RPA Basic Kit (Gen-3), RPA RT Basic Kit (Gen-3), RPA Fluorescent Kit (Gen-3), RPA RT Fluorescent Kit (Gen-3)
• Gen-4: RPA Multiplex Fluorescent Lyophilized Kit (Gen-4)

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory